Identification of a Vitis vinifera endo‐β‐1,3‐glucanase with antimicrobial activity against Plasmopara viticola

• DOI: 10.1111/mpp.12431
• Publication Date: 2016-05-24T16:49:00Z
• Authors: Pere Mestre; Gautier Arista; Marie‐Christine P...; Camille Rustenholz; Christophe Ritzen...; Didier Merdinoglu; Jean‐François Chich
• Keywords: Plasmopara viticola; Glucanase; Oomycete

Summary Inducible plant defences against pathogens are stimulated by infections and comprise several classes of pathogenesis‐related (PR) proteins. Endo‐β‐1,3‐glucanases (EGases) belong to the PR‐2 class their expression is induced many pathogenic fungi oomycetes, suggesting that EGases play a role in hydrolysis pathogen cell walls. However, reports direct effect on walls scarce. Here, we characterized three from Vitis vinifera whose during infection Plasmopara viticola , causal agent downy mildew. Recombinant proteins were expressed Escherichia coli . The enzymatic characteristics these enzymes measured vitro planta A functional assay performed germinated P. spores revealed strong anti‐ activity for EGase3, which strikingly was with lowest catalytic efficiency. To our knowledge, this work shows, first time, mildew family