Glutamate synthase MoGlt1‐mediated glutamate homeostasis is important for autophagy, virulence and conidiation in the rice blast fungus

• DOI: 10.1111/mpp.12541
• Publication Date: 2017-01-31T23:27:29Z
• Authors: Wei Zhou; Wei Shi; Xiao‐Wen Xu; Zhi‐Gang Li; Chang‐Fa Yin; Jun‐Bo Peng; Song Pan; Xiao‐Lin Chen; Wen‐Sheng Zhao; Yan Zhang; Jun Yang; You‐Liang Peng
• Keywords: Conidiation; Appressorium; Glutamate synthase; Homeostasis

Glutamate homeostasis plays a vital role in central nitrogen metabolism and coordinates several key metabolic functions. However, its function fungal pathogenesis development has not been investigated detail. In this study, we identified characterized glutamate synthase gene MoGLT1 the rice blast fungus Magnaporthe oryzae that was important to homeostasis. constitutively expressed, but showed highest expression level appressoria. Deletion of resulted significant reduction conidiation virulence. The ΔMoglt1 mutants were defective appressorial penetration differentiation spread invasive hyphae penetrated plant cells. addition exogenous glutamic acid partially rescued defects infection. Assays for MoAtg8 localization autophagy. delayed mobilization glycogens lipid bodies from conidia developing Taken together, our results show MoGlt1-mediated is fungus, possibly via regulation