Haustoria – arsenals during the interaction between wheat and Puccinia striiformis f. sp. tritici

• DOI: 10.1111/mpp.12882
• Publication Date: 2019-11-27T11:38:30Z
• Authors: Qiang Xu; Chunlei Tang; Likun Wang; Congcong Zhao; Zhensheng Kang; Xiaojie Wang
• Keywords: Haustorium; Obligate parasite; Callose

As an obligate parasite, Puccinia striiformis f. sp. tritici (Pst) forms haustoria to obtain nutrients from plant cells for development, and these structures are essential pathogen survival. To better understand the contribution of interactions with host plants, we isolated susceptible wheat leaves infected Pst race CYR31 sequenced their transcriptome as well those urediospores germ tubes, compared three transcriptomes. A total 3524 up-regulated genes were obtained haustoria, which 73 related thiamine biosynthesis, glycolysis lipid metabolic processes. Silencing seven reduced growth development in wheat. More interestingly, 1197 haustorial secreted proteins (HASPs) detected accounting 34% proteins, indicating that HASPs play important roles haustorium-mediated pathogenic progression. Furthermore, 69 able suppress Bax-triggered programmed cell death tobacco. Additionally, 46 significantly callose deposition using type III secretion system. This study identified a large number effectors through sequencing, results revealed components pathways impact colonization indicate functions pathogenicity Pst.