Kinase‐dead mutation: A novel strategy for improving soybean resistance to soybean cyst nematode Heterodera glycines

• DOI: 10.1111/mpp.13168
• Publication Date: 2021-12-01T15:45:53Z
• Authors: Sarbottam Piya; Tracy Hawk; Bhoomi Patel; Logan Baldwin; John H. Rice; C. Neal Stewart; Tarek Hewezi
• Keywords: Soybean cyst nematode

Protein kinases phosphorylate proteins for functional changes and are involved in nearly all cellular processes, thereby regulating almost aspects of plant growth development, responses to biotic abiotic stresses. We generated two independent co-expression networks soybean genes using control stress response gene expression data identified 392 differentially highly interconnected kinase hub among the networks. Of these kinases, 90 were as "syncytium connected hubs", potentially essential activating signalling pathways nematode feeding site. Overexpression wild-type coding sequences five syncytium transgenic hairy roots enhanced susceptibility cyst (SCN; Heterodera glycines) Hg Type 0 (race 3). In contrast, overexpression kinase-dead variants significantly resistance SCN. Additionally, three tested SCN 1.2.5.7 2), highlighting potential approach generate effective durable against a wide range types. Subcellular localization analysis revealed that mutations do not alter protein localization, confirming structure-function kinase-inactive producing loss-of-function phenotypes causing significant decrease susceptibility. Because many conserved various stresses, our identifying their inactivation mutation could be translated tolerance.