The transcription factors VaERF16 and VaMYB306 interact to enhance resistance of grapevine to Botrytis cinerea infection

• DOI: 10.1111/mpp.13223
• Publication Date: 2022-07-13T06:46:14Z
• Authors: Yanxun Zhu; Xiuming Zhang; Qihan Zhang; Shengyue Chai; Wuchen Yin; Min Gao; Zhi Li; Xiping Wang
• Keywords: Bimolecular fluorescence complementation; Methyl jasmonate; Jasmonate; Pseudomonas syringae; Jasmonic acid; Actinidia

Abstract Botrytis cinerea is a fungus that infects cultivated grape ( Vitis vinifera ); the identification and characterization of resistance mechanisms in host great importance for industry. Here, we report transcription factor ethylene‐responsive (ERF) family VaERF16 ) from Chinese wild amurensis ‘Shuang You’) expressed during B . infection response to treatments with hormones ethylene methyl jasmonate. Heterologous overexpression Arabidopsis thaliana substantially enhanced bacterium Pseudomonas syringae DC3000 via salicylic acid jasmonate/ethylene signalling pathways. Yeast two‐hybrid, bimolecular fluorescence complementation, co‐immunoprecipitation assays indicated interacts MYB VaMYB306. Overexpression or VaMYB306 leaves increased caused an up‐regulation defence‐related gene PDF1.2 , which encodes defensin‐like protein. Conversely, silencing either resulted susceptibility B. one‐hybrid dual‐luciferase transcript levels VaPDF1.2 by binding directly GCC box its promoter. Notably, alone did not bind promoter, but VaERF16–VaMYB306 transcriptional complex higher suggesting proteins function through their mutual interaction. Elucidation this regulatory module may be value enhancing grapevine infection.