Glycoside Hydrolase Family 16 Enzyme RsEG146 From Rhizoctonia solaniAG1 IA Induces Cell Death and Triggers Defence Response in Nicotiana tabacum

• DOI: 10.1111/mpp.70075
• Publication Date: 2025-03-17T07:25:31Z
• Authors: Chen Chen; Dongyang Jiang; Xi Li; Xue Ji; Rui Yang; Yuwen Chen; Ying Chen; Shimin Zuo; Xijun Chen
• Keywords: Pseudomonas syringae; Hypersensitive response

ABSTRACT Rhizoctonia solani AG1 IA is a harmful necrotrophic fungus responsible for various crop diseases, including maize and rice sheath blight, which can lead to significant production losses. However, the pathogenic mechanisms roles of effectors in this pathogen remain poorly understood. In study, we identified glycoside hydrolase 16 family gene, RsEG146 , from R. that was upregulated during its infection Zea mays leaves. When transiently expressed through agroinfiltration, induced cell death leaves tobacco ( Nicotiana tabacum ‘Samsun’). The predicted signal peptide essential death‐inducing activity, while conserved enzymic active site not required. chitin‐binding domain critical activity RsEG146, with Gly47 as key residue. Substitution aspartate, glutamate, or proline significantly impaired RsEG146. Additionally, transient heterogeneous expression enhanced pathogenicity Botrytis cinerea on tobacco, silencing gene spray‐induced (SIGS) reduced severity disease maize, indicating functions an effector. Furthermore, triggered plant immune response tobacco. This study demonstrates potential effector plays crucial role interactions between host.