Insecticide susceptibility status and major detoxifying enzymes activity in Anopheles subpictus from Kasur, Pakistan

• DOI: 10.1111/mve.12367
• Publication Date: 2019-02-19T06:36:26Z
• Authors: H. Naeem; M. Oneeb; K. Ashraf; M. I. Rashid; M. M. Nazir; S. Tabassum
• Keywords: Insecticide Resistance

Anopheles subpictus s.l. Grassi (Diptera: Culicidae) is a malaria vector in South Asia, where insecticides are the mainstay for control interventions. Information on any variation metabolic enzyme levels mosquitoes helpful with respect to adapting alternative strategies control. The scarce data biochemical basis of insecticide resistance vectors Pakistan limit available information interventions within country. susceptibility status and its against dichlorodiphenyltrichloroethane (DDT) (4%), deltamethrin (0.05%) permethrin (0.75%) An. collected from all Tehsils district Kasur were evaluated. For this purpose, World Health Organization bioassay was performed followed by detection altered activity using assays. Similarly, significant difference knock-down effect observed among field susceptible strain 24 h post exposure. overall mean mortality rates DDT, 27.86% [95% confidence interval (CI) = 29.65-26.06], 44.89% (95% CI 46.23-43.54) 78.82% 80.16-77.47), respectively. assays revealed an elevated level enzymes population. results provide evidence organochlorine pyrethroid groups population mediated multiple mechanisms, including acetylcholinesterases, esterases, cytochrome P450 glutathione S-transferases.