Single‐locus DNA barcoding and species delimitation of the sandfly subgenus Evandromyia (Aldamyia)

• DOI: 10.1111/mve.12458
• Publication Date: 2020-06-27T09:51:35Z
• Authors: B. L. Rodrigues; L. A. Baton; P. H. F. Shimabukuro
• Keywords: Subgenus; DNA Barcoding; Sandfly

Abstract Sandfly specimens from the subgenus Evandromyia ( Aldamyia ) Galati, 2003 (Diptera: Psychodidae: Phlebotominae) were collected between 2012 and 2019 nine localities in seven Brazilian states, morphologically‐identified, then DNA barcoded by sequencing mitochondrial cytochrome c oxidase subunit I coi gene. Forty‐four new barcode sequences generated 10 morphospecies combined with 49 previously published same analysed using sequence‐similarity methods (best‐match criteria) to assess their ability at specimen identification, while four different species delimitation (ABGD, GMYC, PTP TCS) used infer molecular operational taxonomic units (MOTUs). Overall, of 11 congruent both well‐supported clades identified phylogenetic analysis MOTUs inferred delimitation, remaining – E. carmelinoi , evandroi lenti piperiformis merged into a single clade/MOTU. Although indistinguishable barcodes, did form distinct cluster could be correctly best‐match criteria. Despite apparent morphological differences, we propose on basis similarity barcodes that these latter should considered members recently‐diverged complex.