Fusarium keratitis in South India: causative agents, their antifungal susceptibilities and a rapid identification method for the Fusarium solani species complex

• DOI: 10.1111/myc.12062
• Publication Date: 2013-02-26T12:13:56Z
• Authors: Mónika Homa; Coimbatore S. Sho...; Yendrembam R. B. ...; Palanisamy Manika...; Kanesan P. Selvam; László Kredics; Venkatapathy Nare...; Csaba Vágvölgyi; László Galgóczy
• Keywords: Natamycin; Terbinafine; Broth microdilution; Species complex; Posaconazole; Fusarium solani; Econazole; Clotrimazole

Seventy Fusarium isolates derived from human keratomycosis were identified based on partial sequences of the β-tubulin (β-TUB) and translation elongation factor 1α (EF-1α) genes. Most confirmed as members F. solani species complex (75.71%), followed by dimerum (8.57%), fujikuroi oxysporum (4.29%) incarnatum-equiseti (2.86%). A combined phylogenetic tree was estimated including all 70 isolates. Isolates belonging to different complexes formed separate clades. In this study, we also report first isolation napiforme keratomycosis. new method a specific EcoRI restriction site in EF-1α gene developed for rapid identification solani. vitro antifungal susceptibilities seven antifungals determined broth microdilution method. Terbinafine, natamycin amphotericin B proved be most effective drugs, voriconazole. The minimal inhibitory concentrations clotrimazole, econazole itraconazole generally high (≥64 μg ml(-1) ). interactions between two (natamycin terbinafine) checkerboard Synergism (71.8%) or no interaction (28.2%) revealed compounds.