MALDI‐TOF mass spectrometry identification of filamentous fungi in the clinical laboratory

• DOI: 10.1111/myc.12115
• Publication Date: 2013-07-30T03:56:29Z
• Authors: Stéphane Ranque; Anne‐Cécile Normand; Carole Cassagne; Jean‐Benjamin Murat; Nathalie Bourgeois; Frédéric Dalle; Martine Gari‐Tous...; Patrick Fourquet; Marijke Hendrickx; Renaud Piarroux
• Keywords: Identification; Dermatophyte

This study aimed to validate the effectiveness of a standardised procedure for MALDI-TOF mass spectrometry (MS)-based identification on large sample filamentous fungi routinely identified in university hospitals' laboratories. Non-dermatophyte prospectively isolated routine activity five teaching hospitals France were first by conventional methods each laboratory and then MS one centre. DNA sequence-based resolved discrepancies between both methods. In this study, 625 analysed 58 species, 501 (80%) 556 (89%) correctly respectively. Compared with method, dramatically enhanced performance non-Aspergillus 31-61% increase correct rate. conclusion, clinical taxa demonstrates that species is significantly improved compared method. The main limitation possible only if included reference spectra library. Nevertheless, laboratory, provides means attain markedly accurate results identification, which was previously restricted few