MALDI‐TOF mass spectrometry identification of filamentous fungi in the clinical laboratory

• DOI: 10.1111/myc.12115
• Publication Date: 2013-07-30T03:56:29Z
• Authors: Stéphane Ranque; Anne‐Cécile Normand; Carole Cassagne; Jean‐Benjamin Murat; Nathalie Bourgeois; Frédéric Dalle; Martine Gari‐Tous...; Patrick Fourquet; Marijke Hendrickx; Renaud Piarroux
• Keywords: MALDI-TOF; 0301 basic medicine; Arthrodermataceae; filamentous fungi; Fungi; 610; Sequence Analysis, DNA; 3. Good health; Hospitals, University; 03 medical and health sciences; [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology; Logistic Models; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; identification; Humans; France; Prospective Studies; [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology; routine laboratory

SummaryThis study aimed to validate the effectiveness of a standardised procedure for the MALDI‐TOF mass spectrometry (MS)‐based identification on a large sample of filamentous fungi routinely identified in university hospitals' laboratories. Non‐dermatophyte filamentous fungi prospectively isolated in the routine activity of five teaching hospitals in France were first identified by conventional methods in each laboratory and then by MS in one centre. DNA sequence‐based identification resolved discrepancies between both methods. In this study, of the 625 analysed filamentous fungi of 58 species, 501 (80%) and 556 (89%) were correctly identified by conventional methods and MS respectively. Compared with the conventional method, MS dramatically enhanced the performance of the identification of the non‐Aspergillus filamentous fungi with a 31–61% increase in correct identification rate. In conclusion, this study on a large sample of clinical filamentous fungi taxa demonstrates that species identification is significantly improved by MS compared with the conventional method. The main limitation is that MS identification is possible only if the species is included in the reference spectra library. Nevertheless, for the routine clinical laboratory, MS provides the means to attain markedly accurate results in filamentous fungi identification, which was previously restricted to only a few reference laboratories.