Diagnostic value of a nested polymerase chain reaction for diagnosing cutaneous sporotrichosis from paraffin‐embedded skin tissue

• DOI: 10.1111/myc.13004
• Publication Date: 2019-09-13T20:38:02Z
• Authors: Shujiro Hayashi; Tomoko Kaminaga; Akiko Baba; Saiko Koike; Masami Koike; Miki Kanno; Satoko Ishikawa; Kyoujyu Tsukada; Toshihiro Suzuki; Yoichiro Hamasaki; Toshimi Sairenchi; Gen Kobashi; Ken Igawa
• Keywords: Sporothrix schenckii; Gold standard (test)

Summary Background The gold standard for diagnosis of cutaneous sporotrichosis involves the isolation fungus, Sporothrix , by a culture test. Generally, sampling test is performed at same time as skin biopsy under local anaesthesia. However, may occasionally return false negative result. Objective aim our study was to investigate diagnostic value molecular method diagnosing from formalin‐fixed and paraffin‐embedded (FFPE) tissues. Methods Over 30‐year period, we collected 52 cases biopsied specimens that had been positively diagnosed A nested PCR specific detection applied using FFPE tissue template. results were compared with control samples 79 patients other diseases according histopathological, clinical findings cutler Results Of who tested positive on test, all detected PCR. 59 in group, 58 Under conditions, calculated sensitivity this 100%, specificity 98.7% kappa coefficient 0.984 (95% CI: 0.953‐1.000). Conclusions assay used appears be useful tool prompt accurate sporotrichosis. Using method, it would possible diagnose suspected but culturing, pathologically whom not undertaken.