Intratumoural immune cell landscape in germinoma reveals multipotent lineages and exhibits prognostic significance

• DOI: 10.1111/nan.12570
• Publication Date: 2019-06-10T08:55:35Z
• Authors: H. Takami; S. Fukushima; K. Aoki; K. Satomi; K. Narumi; N. Hama; Y. Matsushita; K. Fukuoka; K. Yamasaki; T. Nakamura; A. Mukasa; N. Saito; T. Suzuki; T. Yanagisawa; H. Nakamura; K. Sugiyama; K. Tamura; T. Maehara; M. Nakada; M. Nonaka; A. Asai; K. Yokogami; H. Takeshima; T. Iuchi; Y. Kanemura; K. Kobayashi; M. Nagane; K. Kurozumi; K. Yoshimoto; M. Matsuda; A. Matsumura; Y. Hirose; T. Tokuyama; T. Kumabe; K. Ueki; Y. Narita; S. Shibui; Y. Totoki; T. Shibata; Y. Nakazato; R. Nishikawa; M. Matsutani; K. Ichimura
• Keywords: Germinoma; Cell type

Aims Alterations in microenvironments are a hallmark of cancer, and these alterations germinomas particular significance. Germinoma, the most common subtype central nervous system germ cell tumours, often exhibits massive immune infiltration intermingled with tumour cells. The role cells germinoma, however, remains unknown. Methods We investigated cellular constituents their clinical impacts on prognosis 100 germinoma cases. Results Patients lower content (i.e. higher infiltration) had significantly longer progression‐free survival time than those contents ( P = 0.03). Transcriptome analyses RNA in‐situ hybridization indicated that infiltrating comprised wide variety types, including lymphocytes myelocyte‐lineage High expression CD4 was associated good prognosis, whereas elevated nitric oxide synthase 2 poor prognosis. PD1 (PDCD1) expressed by present (93.8%), PD‐L1 (CD274) found majority examined (73.5%). Conclusions collective data strongly suggest play an important predicting treatment response. Further investigation should lead to additional categorization safely reduce intensity depending tumour/immune balance develop possible future immunotherapies.