Interleukin‐6 trans‐signalling induces vascular endothelial growth factor synthesis partly via Janus kinases‐STAT3 pathway in human mesothelial cells

• DOI: 10.1111/nep.12746
• Publication Date: 2016-02-12T08:12:24Z
• Authors: Xiaoxiao Yang; Aiwu Lin; Na Jiang; Hao Yan; Zhaohui Ni; Jiaqi Qian; Wei Fang
• Keywords: Mesothelium

Interleukin-6 (IL-6) is a vital inflammatory factor in the peritoneal cavity of dialysis (PD) patients. Because intraperitoneal inflammation closely associated with angiogenesis, we sought to explore effect IL-6 on vascular endothelial growth (VEGF) synthesis and its transduction pathway mesothelial cells.Human cells (Met-5A) were incubated different concentrations glucose mannitol, mannitol expression was determined. Then, stimulated by or without two soluble receptors (sIL-6R sgp130), VEGF detected. Finally, IL-6/sIL-6R combined inhibitor Janus kinases (JAK) AG490. The phosphorylation signal transducer activator transcription 3 (STAT3) intracellular translocation examined.1. High could upregulate mRNA secretion significantly, there no difference between high mannitol. 2. Met-5A cell line single receptor. complex induced cells, which alleviated sgp130 trans-signalling induce STAT3, recruited cellular nucleus cells.The present study might provide evidence that upregulates some extent, depending osmolality partly dependent JAK/STAT3 pathway.