Vascular calcification is associated with Wnt‐signaling pathway and blood pressure variability in chronic kidney disease rats

• DOI: 10.1111/nep.13677
• Publication Date: 2019-11-11T08:28:26Z
• Authors: Ruoxi Liao; Liya Wang; Jiameng Li; Si Sun; Yuqin Xiong; Yupei Li; Mei Han; Heng Jiang; Mahajan Anil; Baihai Su
• Keywords: Sclerostin; Osteopontin

Abstract Aim Vascular calcification (VC) is a common complication in chronic kidney disease (CKD) and has been shown to be associated with increased cardiovascular events mortality. This study was explore the role of Wnt‐signaling pathway CKD VC, association between VC blood pressure variability (BPV) which risk factor events. Methods Adult male Sprague‐Dawley rats were divided into adenine‐induced group (n = 5), 5/6 nephrectomy sham 5) control 5). Low‐calcium‐high‐phosphate diets introduced induce vascular calcification. Both daytime (hour‐to‐hour during day) mid‐term (day‐to‐day for 9 days) (BP) collected analyzed BPV metrics. At sacrifice, kidney, heart aorta samples taken histological analyses. Calcium deposition identified Alizarin Red stain graded. Immunohistochemistry western blot performed Wnt3a, Wnt5a, β‐catenin, sclerostin, osteopontin, α‐SMA. Results Compared rats, suffered from markedly severer (Grade 2.6 ± 0.2 1.8 0.8 vs 0.0 0.4, P .0010). positively correlated Wnt3a β‐catenin expression ( .0032 .0000), but not significantly Wnta5a or sclerostin. Besides, showed < .001), also VC. Conclusion We confirmed that had enhanced tissue together BPV. Wnt may potential target future management CKD.