Transient receptor potential ankyrin 1 agonists improve intestinal transit in a murine model of postoperative ileus

• DOI: 10.1111/nmo.12877
• Publication Date: 2016-06-10T08:54:16Z
• Authors: K. Tsuchiya; K. Kubota; K. Ohbuchi; A. Kaneko; N. Ohno; A. Mase; H. Matsushima; M. Yamamoto; K. Miyano; Y. Uezono; T. Kono
• Keywords: Male; 0301 basic medicine; 2. Zero hunger; 0303 health sciences; Mice, 129 Strain; Dose-Response Relationship, Drug; Amides; 3. Good health; Mice, Inbred C57BL; Disease Models, Animal; Mice; 03 medical and health sciences; Ileus; Organ Culture Techniques; Animals; Acrolein; Gastrointestinal Transit; TRPA1 Cation Channel; Cells, Cultured; Naphthoquinones

Abstract Background Stimulation of transient receptor potential ankyrin 1 ( TRPA 1), which abundantly expressed in enterochromaffin cells ECC ), has been reported to exert apparently contradictory results vitro contractility and vivo gastrointestinal GI ) transit evaluations. The pharmaceutical‐grade Japanese traditional medicine daikenchuto TU ‐100) be beneficial for postoperative ileus POI accelerate animals humans. ‐100 was recently shown increase intestinal blood flow via stimulation the epithelial small intestine SI ). Methods effects various agonists on motility were examined a manipulation‐induced murine model, culture segments an model cell line, RIN ‐14B. Key Results Orally administered agonists, aryl isothiocyanate AITC cinnamaldehyde CA ingredients, [6]‐shogaol (6S) γ ‐sanshool GS improved model. , 6S but not abrogated 1‐deficient mice. show periodic peristaltic motor activity whose periodicity disappeared augmented motility. increased 5‐ HT release from isolated all these compounds except lost induced ‐14B dose‐ 1‐dependent manner. Conclusions & Inferences Intraluminal is therapeutic strategy disorders. Further investigation required determine whether and/or are involved effect