Exploration of associations among dietary tryptophan, microbiome composition and function, and symptom severity in irritable bowel syndrome

• DOI: 10.1111/nmo.14545
• Publication Date: 2023-02-13T20:18:47Z
• Authors: Anna M. Plantinga; Kendra J. Kamp; Qinglong Wu; Li Chen; Linda Yoo; Robert L. Burr; Kevin C. Cain; Daniel Raftery; Fausto Carnevale ...; Shyam Badu; Sik Yu So; Tor Savidge; Robert J. Shulman; Margaret M. Heitk...
• Keywords: 2. Zero hunger; Adult; Irritable Bowel Syndrome; 0301 basic medicine; 03 medical and health sciences; Microbiota; Tryptophan; Humans; Female; 3. Good health; Diet; Gastrointestinal Microbiome

AbstractBackgroundImbalance of the tryptophan (TRP) pathway may influence symptoms among patients with irritable bowel syndrome (IBS). This study explored relationships among different components that contribute to TRP metabolism (dietary intake, stool metabolite levels, predicted microbiome metabolic capability) in females with IBS and healthy controls (HCs). Within the IBS group, we also investigated relationships between TRP metabolic determinants, Bifidobacterium abundance, and symptoms of IBS.MethodsParticipants with IBS (Rome III) and HCs completed a 28‐day diary of gastrointestinal symptoms and a 3‐day food record for TRP intake. They provided a stool sample for shotgun metagenomics, 16 S rRNA analyses, and quantitative measurement of TRP by mass spectrometry.ResultsOur cohort included 115 females, 69 with IBS and 46 HCs, with a mean age of 28.5 years (SD 7.4). TRP intake (p = 0.71) and stool TRP level (p = 0.27) did not differ between IBS and HC. Bifidobacterium abundance was lower in the IBS group than in HCs (p = 0.004). Predicted TRP metabolism gene content was higher in IBS than HCs (FDR‐corrected q = 0.006), whereas predicted biosynthesis gene content was lower (q = 0.045). Within the IBS group, there was no association between symptom severity and TRP intake or stool TRP, but there was a significant interaction between Bifidobacterium abundance and TRP intake (q = 0.029) in predicting stool character.ConclusionsDietary TRP intake, microbiome composition, and differences in TRP metabolism constitute a complex interplay of factors that could modulate IBS symptom severity.