RoKSN, a floral repressor, forms protein complexes with RoFD and RoFT to regulate vegetative and reproductive development in rose

• DOI: 10.1111/nph.12625
• Publication Date: 2013-12-06T09:36:41Z
• Authors: Marie Randoux; Jean‐Michel Davière; Julien Jeauffre; Tatiana Thouroude; Sandrine Pierre; Youness Toualbia; Justine Perrotte; Jean‐Paul Reynoird; Marie‐José Jammes; Laurence Hibrand‐...; Fabrice Foucher
• Keywords: [SDV]Life Sciences [q-bio]; Arabidopsis; Flowers; Genes, Plant; Rosa; 03 medical and health sciences; Gene Expression Regulation, Plant; RNA, Messenger; Inflorescence; polycarpic; rose; transgenic; Plant Proteins; 580; 0303 health sciences; TERMINAL FLOWER 1 (TFL1); PEBP family; Arabidopsis Proteins; Reproduction; Genetic Complementation Test; Gene Expression Regulation, Developmental; [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology; 15. Life on land; Plants, Genetically Modified; FLOWERING LOCUS T(FT); Repressor Proteins; Mutation

Summary FT / TFL 1 family members have been known to be involved in the development and flowering plants. In rose, R o KSN , a homologue, is key regulator of flowering, whose absence causes continuous flowering. Our objectives are functionally validate explore its mode action rose. We complemented A rabidopsis tfl1 mutants ectopically expressed continuous‐flowering (CF) Using different protein interaction techniques, we studied interactions with FD possible competition. Arabidopsis mutant by rescuing late indeterminate growth. CF roses, ectopic expression led Different branching patterns were observed some transgenic plants had an increased number leaflets per leaf. these floral activator transcripts decreased. Furthermore, was able interact both activator, . Protein experiments revealed that could compete for repression activation blooming, respectively. conclude repressor also vegetative forms complex