Salicylic acid binding of mitochondrial alpha‐ketoglutarate dehydrogenase E2 affects mitochondrial oxidative phosphorylation and electron transport chain components and plays a role in basal defense against tobacco mosaic virus in tomato

• DOI: 10.1111/nph.13137
• Publication Date: 2014-11-03T13:46:06Z
• Authors: Yangwenke Liao; Miaoying Tian; Huan Zhang; Xin Li; Yu Wang; Xiaojian Xia; Jie Zhou; Yanhong Zhou; Jingquan Yu; Kai Shi; Daniel F. Klessig
• Keywords: Alternative oxidase

Summary Salicylic acid ( SA ) plays a critical role in plant defense against pathogen invasion. ‐induced viral plants is distinct from the pathways mediating bacterial and fungal involves specific pathway mediated by mitochondria; however, underlying mechanisms remain largely unknown. The SA‐binding activity of recombinant tomato Solanum lycopersicum alpha‐ketoglutarate dehydrogenase Sl α‐k GDH E2 subunit tricarboxylic (TCA) cycle was characterized. biological this binding defenses tobacco mosaic virus (TMV) further investigated via α ‐k silencing transient overexpression plants. α‐ kGDH found to bind two independent assays. treatment, as well silencing, increased resistance TMV. did not enhance TMV ‐silenced but reduce susceptibility Nicotiana benthamiana transiently overexpressing . Furthermore, ‐silencing‐induced fully blocked bongkrekic application alternative oxidase 1a silencing. These results indicated that acts upstream affects mitochondrial electron transport chain, which an important basal findings study help elucidate SA‐induced defense.