Grassland species differentially regulate proline concentrations under future climate conditions: an integrated biochemical and modelling approach
Ornithine
0301 basic medicine
2. Zero hunger
Proline
Research
Climate
Glutamic Acid
15. Life on land
Grassland
Models, Biological
03 medical and health sciences
Species Specificity
13. Climate action
Thermodynamics
Pyrroles
RNA, Messenger
Biology
Monte Carlo Method
Metabolic Networks and Pathways
DOI:
10.1111/nph.13481
Publication Date:
2015-06-03T03:24:15Z
AUTHORS (6)
ABSTRACT
Summary
Proline (Pro) is a versatile metabolite playing a role in the protection of plants against environmental stresses. To gain a deeper understanding of the regulation of Pro metabolism under predicted future climate conditions, including drought stress, elevated temperature and CO2, we combined measurements in contrasting grassland species (two grasses and two legumes) at multiple organisational levels, that is, metabolite concentrations, enzyme activities and gene expression.
Drought stress (D) activates Pro biosynthesis and represses its catabolism, and elevated temperature (DT) further elevated its content. Elevated CO2 attenuated the DT effect on Pro accumulation.
Computational pathway control analysis allowed a mechanistic understanding of the regulatory changes in Pro metabolism. This analysis indicates that the experimentally observed coregulation of multiple enzymes is more effective in modulating Pro concentrations than regulation of a single step. Pyrroline‐5‐carboxylate synthetase (P5CS) and pyrroline‐5‐carboxylate reductase (P5CR) play a central role in grasses (Lolium perenne, Poa pratensis), and arginase (ARG), ornithine aminotransferase (OAT) and P5CR play a central role in legumes (Medicago lupulina, Lotus corniculatus).
Different strategies in the regulation of Pro concentrations under stress conditions were observed. In grasses the glutamate pathway is activated predominantly, and in the legumes the ornithine pathway, possibly related to differences in N‐nutritional status.
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