Comparative genomics, proteomics and transcriptomics give new insight into the exoproteome of the basidiomycete Hebeloma cylindrosporum and its involvement in ectomycorrhizal symbiosis

• DOI: 10.1111/nph.13546
• Publication Date: 2015-07-14T15:11:42Z
• Authors: Jeanne Doré; Marie Perraud; Cindy Dieryckx; Annegret Kohler; Emmanuelle Morin; Bernard Henrissat; Erika Lindquist; Sabine D. Zimmermann; Vincent Girard; Alan Kuo; Igor V. Grigoriev; Francis Martin; Roland Marmeisse; Gilles Gay
• Keywords: Proteome; Comparative Genomics

Summary Extracellular proteins play crucial roles in the interaction between mycorrhizal fungi and their environment. Computational prediction experimental detection allowed identification of 869 constituting exoproteome Hebeloma cylindrosporum . Small secreted ( SSP s) carbohydrate‐active enzymes CAZ ymes) were two major classes extracellular proteins. Twenty‐eight per cent s by free‐living mycelia five 10 most abundant s. By contrast, 63–75% involved nutrient acquisition secreted. A total 150 protein‐coding genes differentially expressed mycorrhizas mycelia. affected. External environmental conditions also affected expression 199 mycorrhizas. displayed different patterns regulation response to presence a host plant or other signals. Several overexpressed organic matter encoded oxidoreductases. has not fully lost its ancestral saprotrophic capacities but rather adapted them harm hosts use soil nitrogen. The complex divergent symbiotic partner and/or suggest various biology fungi.