Regulation of anthocyanin and proanthocyanidin biosynthesis by Medicago truncatula bHLH transcription factor MtTT8

• DOI: 10.1111/nph.13816
• Publication Date: 2016-01-04T10:31:03Z
• Authors: Penghui Li; Beibei Chen; Gaoyang Zhang; Longxiang Chen; Qiang Dong; Jiangqi Wen; Kirankumar S. Mysore; Jian Zhao
• Keywords: Medicago truncatula

Summary The MYB ‐ basic helix–loop–helix ( bHLH )‐ WD 40 complexes regulating anthocyanin and proanthocyanidin PA ) biosynthesis in plants are not fully understood. Here Medicago truncatula Mt TT 8 was characterized as a central component of these ternary that control biosynthesis. Mttt8 mutant seeds have transparent testa phenotype with reduced s anthocyanins. restores productions Arabidopsis tt8 mutant. Ectopic expression anthocyanins mttt8 plant hairy roots further enhances both wild‐type roots. Transcriptomic analyses metabolite profiling M. mutant, complemented , or overexpression lines) indicate regulates subset genes involved is genetically regulated by LAP 1, PAR 40‐1. Combinations 40‐1 activate promoter yeast assay. interacts transcription factors to form regulatory complexes. 8, an factor, interacted activated promoters anthocyanidin reductase synthase regulate biosynthesis, respectively. Our results provide new insights into the complex regulation .