Molecular speciation and tissue compartmentation of zinc in durum wheat grains with contrasting nutritional status

• DOI: 10.1111/nph.13989
• Publication Date: 2016-05-09T16:25:04Z
• Authors: Daniel Pergament ...; Thomas C. de Bang; Pai R. Pedas; Umit Baris Kutman; Ismail Cakmak; Birgit Andersen; Christine Finnie; Jan K. Schjoerring; Søren Husted
• Keywords: Genetic algorithm; Biofortification

Summary Low concentration of zinc (Zn) in the endosperm cereals is a major factor contributing to Zn deficiency human populations. We have investigated how combined and nitrogen (N) fertilization affects speciation localization durum wheat ( Triticum ). Zn‐binding proteins were analysed with liquid chromatography ICP ‐ MS Orbitrap 2 , respectively. Laser ablation simultaneous Zn, sulphur (S) phosphorus (P) detection was used for bioimaging its potential ligands. Increasing N supply had impact on endosperm, reaching concentrations higher than current breeding targets. The S also increased, but only partly co‐localized Zn. mutual enrichment reflected substantially more bound small cysteine‐rich (apparent size 10–30 kDa), whereas response larger > 50 kDa) modest. Most Zn‐responsive associated redox‐ stress‐related processes. This study offers methodological platform deepen understanding processes behind enrichment. Novel information provided modified during biofortification grains.