ETHYLENE RESPONSE FACTOR 74 (ERF74) plays an essential role in controlling a respiratory burst oxidase homolog D (RbohD)‐dependent mechanism in response to different stresses in Arabidopsis

• DOI: 10.1111/nph.14278
• Publication Date: 2016-11-07T11:11:53Z
• Authors: Yuan Yao; Run Jun He; Qiao Li Xie; Xian hai Zhao; Xiao mei Deng; Jun bo He; Lili Song; Jun He; Alan Marchant; Xiao‐Yang Chen; Ai‐Min Wu
• Keywords: Chromatin immunoprecipitation

Summary Recent studies indicate that the ETHYLENE RESPONSE FACTOR VII (ERF‐VII) transcription factor is an important regulator of osmotic and hypoxic stress responses in plants. However, molecular mechanism ERF‐VII‐mediated transcriptional regulation remains unclear. Here, we investigated role ERF74 (a member ERF‐VII protein family) by examining abiotic tolerance overexpression line a T‐DNA insertion mutant using flow cytometry, transactivation electrophoretic mobility shift assays. 35S:: showed enhanced to drought, high light, heat aluminum stresses, whereas erf74 erf74;erf75 double displayed higher sensitivity. Using cytometry analysis, found lines lack reactive oxygen species (ROS) burst early stages various as result lower expression level RESPIRATORY BURST OXIDASE HOMOLOG D ( RbohD ). Furthermore, directly binds promoter activates its under different stresses. Moreover, induction marker genes ROS‐scavenging enzyme conditions dependent on − RbohD− ROS signal pathway. We propose pathway involves acting on–off switch controlling ‐dependent response subsequently maintaining hydrogen peroxide (H 2 O ) homeostasis Arabidopsis.