Respiration climacteric in tomato fruits elucidated by constraint‐based modelling

• DOI: 10.1111/nph.14301
• Publication Date: 2016-11-16T18:19:06Z
• Authors: Sophie Colombié; Bertrand Beauvoit; Christine Nazaret; Camille Bénard; Gilles Vercambre; Sophie Le Gall; Benoit Biais; Cécile Cabasson; Mickaël Maucourt; Stéphane Bernillon; Annick Moing; Martine Dieuaide‐...; Jean‐Pierre Mazat; Yves Gibon
• Keywords: Climacteric

Summary Tomato is a model organism to study the development of fleshy fruit including ripening initiation. Unfortunately, few studies deal with brief phase accelerated associated respiration climacteric because practical problems involved in measuring respiration. Because constraint‐based modelling allows predicting accurate metabolic fluxes, we investigated and energy dissipation pericarp at breaker stage using detailed stoichiometric respiratory pathway, alternative oxidase uncoupling proteins. Assuming steady‐state, dataset was transformed into constraints solve on daily basis throughout tomato development. We detected peak CO 2 released an excess dissipated 40 d post anthesis ( DPA ) just before onset coinciding climacteric. demonstrated unbalanced carbon allocation sharp slowdown accumulation (for syntheses storage) beginning degradation starch cell wall polysaccharides. Experiments fruits harvested from plants cultivated under stress conditions confirmed concept. conclude that efficient tool bypass difficulty elucidate underlying mechanisms ripening.