The pentatricopeptide repeat protein EMP9 is required for mitochondrial ccmB and rps4 transcript editing, mitochondrial complex biogenesis and seed development in maize

• DOI: 10.1111/nph.14424
• Publication Date: 2017-01-25T14:07:08Z
• Authors: Yan‐Zhuo Yang; Shuo Ding; Hong‐Chun Wang; Feng Sun; Wen‐Long Huang; Shu Song; Chunhui Xu; Bao‐Cai Tan
• Keywords: Pentatricopeptide repeat

Pentatricopeptide repeat (PPR) proteins comprise a large family of sequence-specific RNA binding in land plants. Because its size and frequent embryo lethality the mutants, molecular functions physiological roles many PPR are unknown. Through characterization an empty pericarp9 (emp9) mutant maize (Zea mays), we defined EMP9 mitochondrial editing, respiratory complex formation seed development. Mu insertions different regions Emp9 facilitated dissection domain EMP9. genetic functional analyses multiple alleles, showed that deletions two N-terminal motifs partial E+ do not eliminate editing function encodes subclass protein is localized mitochondria. Loss abolishes C-to-U ccmB-43 rps4-335 sites The loss at affects maturation cytochrome c impairs biogenesis complexes, particularly III. This work extends our understanding PPR-E+ development, provides insights into CcmB higher