The iron‐regulated transporter 1 plays an essential role in uptake, translocation and grain‐loading of manganese, but not iron, in barley

• DOI: 10.1111/nph.14930
• Publication Date: 2017-12-05T13:50:44Z
• Authors: Lizhi Long; Daniel P. Persson; Fengying Duan; Kirsten Jørgensen; Lixing Yuan; Jan K. Schjoerring; Pai R. Pedas
• Keywords: Endodermis; Hydroponics; Stele

Summary Transporters involved in manganese (Mn) uptake and intracellular Mn homeostasis Arabidopsis rice are well characterized, while much less is known for barley, which particularly prone to deficiency. In this study we have investigated the role of iron‐regulated transporter 1 ( IRT 1) translocation barley plants. We employed an RNA i approach reduce Hv expression 5% wild‐type level. This enabled characterization functional by use advanced imaging phenotyping techniques applied plants growing hydroponics or soils with different availability. Our results highlight importance transport across root endodermis into stele. hvirt1 ‐ lines, a chlorotic phenotype reduced shoot concentration impaired photosynthetic functionality was observed, especially under conditions low also document that controlled distribution within grain. Surprisingly, unlike other orthologues, played no significant iron uptake. conclude orthologue has novel function respect ensuring sufficient concentrations. The preference instead Fe discussed evolutionary context.