Rice PPS1 encodes a DYW motif‐containing pentatricopeptide repeat protein required for five consecutive RNA‐editing sites of nad3 in mitochondria

• DOI: 10.1111/nph.15347
• Publication Date: 2018-07-19T05:38:42Z
• Authors: Haijun Xiao; Qiannan Zhang; Xiaojian Qin; Yanghong Xu; Chenzi Ni; Jishuai Huang; Linlin Zhu; Feiya Zhong; Wei Liu; Guoxin Yao; Yingguo Zhu; Jun Hu
• Keywords: Pentatricopeptide repeat; Organelle; Cytoplasmic male sterility

The pentatricopeptide repeat (PPR) protein family is a large characterized by tandem arrays of degenerate 35-amino-acid motif whose members function as important regulators organelle gene expression at the post-transcriptional level. Despite roles PPRs in RNA editing organelles, their activities and underlying mechanism remain obscure. Here, we show that novel DYW motif-containing PPR protein, PPS1, associated with five conserved RNA-editing sites nad3 located close proximity to each other mitochondria, all which involve conversion from proline leucine rice. Both pps1 RNAi heterozygous plants are delayed development partial pollen sterility vegetative stages reproductive stage. electrophoresis mobility shift assays (REMSAs) reciprocal competition using different versions probes confirm PPS1 can bind cis-elements near affected sites, distinct existing mode PPR-RNA binding because continuity sites. Loss reduces activity several complexes mitochondrial electron transport chain affects morphology. Taken together, our results indicate required for specific