Sclerenchyma cell thickening through enhanced lignification induced by OsMYB30 prevents fungal penetration of rice leaves

• DOI: 10.1111/nph.16505
• Publication Date: 2020-02-29T11:30:09Z
• Authors: Weitao Li; Kang Wang; Mawsheng Chern; Yuchen Liu; Ziwei Zhu; Jiang Liu; Xiaobo Zhu; Junjie Yin; Li Ran; Jun Xiong; Kaiwei He; Liting Xu; Min He; Jing Wang; Jiali Liu; Yu Bi; Hai Qing; Mingwu Li; Kun Hu; Li Song; Long Wang; Tuo Qi; Qingqing Hou; Weilan Chen; Yan Li; Wenming Wang; Xuewei Chen
• Keywords: Magnaporthe; Pyricularia

Broad-spectrum resistance is highly preferred in crop breeding programmes. Previously, we have reported the identification of broad-spectrum resistance-Digu 1 (bsr-d1) allele from rice Digu. The bsr-d1 prevents activation Bsr-d1 expression by Magnaporthe oryzae infection and degradation H2 O2 peroxidases, leading to M. oryzae. However, it remains unknown whether defence pathways other than burst peroxidases contribute bsr-d1-mediated immunity. Blast was determined leaves spray punch inoculations. Target genes OsMYB30 were identified one-hybrid assays yeast electrophoretic mobility shift assay. Lignin content measured phloroglucinol-HCl staining, acetyl bromide thioacidolysis methods. Here, report involvement gene blast resistance. Expression induced during or when knocked out downregulated, as occurs plants upon infection. We further found that bound activated promoters 4-coumarate:coenzyme A ligase (Os4CL3 Os4CL5) resulting accumulation lignin subunits G S. This action led obvious thickening sclerenchyma cells near epidermis, inhibiting penetration at early stage Our study revealed novel components required for penetration-dependent immunity, advanced our understanding disease