Sniffing fungi – phenotyping of volatile chemical diversity inTrichodermaspecies

• DOI: 10.1111/nph.16530
• Publication Date: 2020-03-10T23:25:56Z
• Authors: Yuan Guo; Werner Jud; Andrea Ghirardo; Felix Antritter; J. Philipp Benz; Jörg‐Peter Schnit...; Maaria Rosenkranz
• Keywords: Sniffing; Trichoderma; Volatile organic compound

Summary Volatile organic compounds (VOCs) play vital roles in the interaction of fungi with plants and other organisms. A systematic study global fungal VOC profiles is still lacking, though it a prerequisite for elucidating mechanisms VOC‐mediated interactions. Here we present versatile system enabling high‐throughput screening VOCs under controlled temperature. In proof‐of‐principle experiment, characterized volatile metabolic fingerprints four Trichoderma spp. over 48 h growth period. The developed platform allows automated fast detection from up to 14 simultaneously growing cultures real time. comprehensive analysis odors achieved by employing proton transfer reaction‐time flight‐MS GC‐MS. data‐mining strategy based on multivariate data machine learning be uncovered. Our revealed dynamic, development‐dependent extremely species‐specific biocontrol genus . two mass spectrometric approaches were highly complementary each other, together revealing novel, dynamic view release. This analytical could used VOC‐based chemotyping diverse small organisms, or more generally, any vivo vitro real‐time headspace analysis.