Silencing the alarm: an insect salivary enzyme closes plant stomata and inhibits volatile release

• DOI: 10.1111/nph.17214
• Publication Date: 2021-01-18T14:28:40Z
• Authors: Po‐An Lin; Yintong Chen; Duverney Chaverra...; Chan Chin Heu; Nursyafiqi Bin Za...; Jagdeep Singh Sidhu; Michelle Peiffer; Ching‐Wen Tan; Anjel Helms; Donghun Kim; Jared Ali; Jason L. Rasgon; Jonathan Lynch; Charles T. Anderson; Gary W. Felton
• Keywords: Methyl salicylate; Green leaf volatiles

Summary Herbivore‐induced plant volatiles (HIPVs) are widely recognized as an ecologically important defensive response of plants against herbivory. Although the induction this ‘cry for help’ has been well documented, only a few studies have investigated inhibition HIPVs by herbivores and little is known about whether evolved mechanisms to inhibit release HIPVs. To examine role herbivore effectors in modulating stomatal dynamics, we conducted series experiments combining pharmacological, surgical, genetic (CRISPR‐Cas9) chemical (GC‐MS analysis) approaches. We show that salivary enzyme, glucose oxidase (GOX), secreted caterpillar Helicoverpa zea on leaves, causes closure tomato ( Solanum lycopersicum ) within 5 min, both soybean Glycine max at least 48 h. GOX also inhibits emission several during feeding H. , including Z )‐3‐hexenol, )‐jasmone )‐3‐hexenyl acetate, which airborne signals defenses. Our findings highlight potential adaptive strategy where insect defenses exploiting association between dynamics HIPV emission.