Carrot mottle virus ORF4 movement protein targets plasmodesmata by interacting with the host cell SUMOylation system

• DOI: 10.1111/nph.17370
• Publication Date: 2021-03-28T16:36:35Z
• Authors: Jun Jiang; Yen‐Wen Kuo; Nidà Salem; Anna Erickson; Bryce W. Falk
• Keywords: Plasmodesma; Movement protein

Plant virus movement proteins (MPs) facilitate spread in their plant hosts, and some of them are known to target plasmodesmata (PD). However, how the MPs PD is still largely unknown. Carrot mottle (CMoV) encodes ORF3 ORF4 proteins, which involved CMoV movement. In this study, we used as a model study targeting MP. We showed that protein, but not modified led found protein interacts with host cell small ubiquitin-like modifier (SUMO) 1, 2 SUMO-conjugating enzyme SCE1, resulting SUMOylation. Downregulation mRNAs for NbSCE1 NbSUMO impaired infection. The SUMO-interacting motifs (SIMs) LVIVF, VIWV, lysine residue at position 78 (K78) required mutation these prevented efficiently PD, further slowed or completely abolished systemic Finally, highly conserved among umbraviruses. Our data suggest targets by interacting SUMOylation system.