Aldoximes are precursors of auxins in Arabidopsis and maize

• DOI: 10.1111/nph.17447
• Publication Date: 2021-05-08T14:01:48Z
• Authors: Veronica C. Perez; Ru Dai; Bing Bai; Breanna Tomiczek; Bryce C. Askey; Yi Zhang; Garret M. Rubin; Yousong Ding; Alexander Grenning; Anna K. Block; Jeongim Kim
• Keywords: Indole-3-acetic acid

Two natural auxins, phenylacetic acid (PAA) and indole-3-acetic (IAA), play crucial roles in plant growth development. One route of IAA biosynthesis uses the glucosinolate intermediate indole-3-acetaldoxime (IAOx) as a precursor, which is thought to occur only glucosinolate-producing plants Brassicales. A recent study showed that overproducing phenylacetaldoxime (PAOx) Arabidopsis increases PAA production. However, it remains unknown whether this increased resulted from hydrolysis PAOx-derived benzyl or, like IAOx-derived IAA, directly converted PAOx. If not required, aldoxime-derived auxin may beyond To better understand biosynthesis, we conducted an isotope-labelled aldoxime feeding assay using glucosinolate-deficient mutant sur1 maize, transcriptomics analysis. Our demonstrated conversion PAOx does require glucosinolates Arabidopsis. Furthermore, maize produces IAOx, respectively, indicating also occurs maize. Considering production widely kingdom, likely be more widespread than originally believed. genome-wide PAOx-overproduction identified complex metabolic networks among PAA, phenylpropanoid tryptophan metabolism.