An aspartic protease 47 causes quantitative recessive resistance to rice black‐streaked dwarf virus disease and southern rice black‐streaked dwarf virus disease

• DOI: 10.1111/nph.17961
• Publication Date: 2022-01-11T18:30:58Z
• Authors: Zhaoyun Wang; Lian Zhou; Ying Lan; Xuejuan Li; Jian Wang; Jingfang Dong; Wei Guo; Dedao Jing; Qing Liu; Shaohong Zhang; Zhiyang Liu; Wenjuan Shi; Wu Yang; Tifeng Yang; Feng Sun; Linlin Du; Hua Fu; Yamei Ma; Yudong Shao; Luo Chen; Jitong Li; Shuo Li; Yongjian Fan; Yunyue Wang; Hei Leung; Bin Liu; Yijun Zhou; Junliang Zhao; Tong Zhou
• Keywords: Candidate gene; Black rice

Rice black-streaked dwarf virus disease (RBSDVD) and southern rice (SRBSDVD) are the most destructive viral diseases in rice. Progress is limited breeding due to lack of resistance resource inadequate knowledge on underlying functional gene. Using genome-wide association study (GWAS), linkage disequilibrium (LD) decay analyses, RNA-sequencing, genome editing, we identified a highly RBSDVD-resistant variety its first A W44 was through extensive evaluation diverse international panel. Seventeen quantitative trait loci (QTLs) were among which qRBSDV6-1 had largest phenotypic effect. It finely mapped 0.8-1.2 Mb region chromosome 6, with 62 annotated genes. Analysis candidate genes showed high expression aspartic proteinase 47 (OsAP47) susceptible variety, W122, low W44. OsAP47 overexpressing lines exhibited significantly reduced resistance, while knockout mutants SRBSDVD RBSDVD severity. Furthermore, resistant allele Hap1 almost exclusive Indica, but rare Japonica. Results suggest that by editing effective for improving resistance. This provides genetic information cultivars.