OsADK1, a novel kinase regulating arbuscular mycorrhizal symbiosis in rice

• DOI: 10.1111/nph.17979
• Publication Date: 2022-02-08T10:18:43Z
• Authors: Rui Guo; Ya‐Nan Wu; Cheng‐Chen Liu; Ying‐Na Liu; Li Tian; Jian‐Fei Cheng; Zhiyong Pan; Dong Wang; Bin Wang
• Keywords: Arbuscular mycorrhizal fungi

Summary Arbuscular mycorrhizal (AM) symbiosis relies on the formation of arbuscules for efficient nutrient exchange between plants and AM fungi. In this study, we identified a novel kinase gene in rice named OsADK1 ( Arbuscule Development Kinase 1 ) that is required arbuscule development. By obtaining OsADK1pro :: GUS transgenic also generating Osadk1 mutants via CRISPR/Cas9 technique, was revealed to be specifically induced arbusculated cortical cells mutations resulted an extremely low colonisation rate c . 3%) roots by fungus Rhizophagus irregularis mutant roots, very few observed nearly all arrested at early ‘trunk‐forming’ phase without forming any branches. Increasing inoculum strength or cocultivation with wild‐type nurse plant did not result rescue phenotype. Transcriptome sequencing both nursed un‐nursed then mutation could greatly affect symbiotic programme, including many key transcription factors such as RAM1 WRI5. therefore represents new branching. Its identification opens window explore elaborate signal transduction pathway determines development during plant–fungus symbiosis.