Phylogenetic distribution and expression pattern analyses identified a divergent basal body assembly protein involved in land plant spermatogenesis

• DOI: 10.1111/nph.18385
• Publication Date: 2022-07-17T06:30:12Z
• Authors: Shizuka Koshimizu; Naoki Minamino; Tomoaki Nishiyama; Emiko Yoro; Mayuko Sato; Mayumi Wakazaki; Kiminori Toyooka; Kazuo Ebine; Keiko Sakakibara; Takashi Ueda; Kentaro Yano
• Keywords: Marchantia polymorpha; Basal body; Spermiogenesis

Land plant spermatozoids commonly possess characteristic structures such as the spline, which consists of a microtubule array, multilayered structure (MLS) in uppermost layer is continuum and multiple flagella. However, molecular mechanisms underpinning spermatogenesis remain to be elucidated. We successfully identified candidate genes involved spermatogenesis, deeply divergent BLD10s, by computational analyses combining methods omics data. then examined functions BLD10s liverwort Marchantia polymorpha moss Physcomitrium patens. MpBLD10 PpBLD10 are required for normal basal body (BB) flagella formation. Mpbld10 mutants exhibited defects remodeling cytoplasm nucleus during spermatozoid formation, thus should chromatin reorganization elimination spermiogenesis. orthologs diverse Streptophyta found that orthologous BLD10/CEP135 family proteins, function BB assembly. evolved especially quickly land plants might have acquired additional formation through rapid evolution.