Nutrient status regulates MED19a phase separation for ORESARA1‐dependent senescence

• DOI: 10.1111/nph.18478
• Publication Date: 2022-09-12T08:41:59Z
• Authors: Steven Le Hung Cheng; Hui‐Wen Wu; Haiying Xu; Reuben Manjit Singh; Tao Yao; In‐Cheol Jang; Nam‐Hai Chua
• Keywords: Senescence

The mediator complex is highly conserved in eukgaryotes and integral for transcriptional responses. Mediator subunits associate with signal-responsive transcription factors (TF) to activate expression of specific genes. As the key TF Arabidopsis thaliana senescence, ORESARA1 (ORE1) required nitrogen deficiency (-N) induced senescence; however, subunit that associates ORE1 remains unknown. Here, we show MED19a -N senescence-responsive Disordered forms inducible nuclear condensates under regulated by decreasing lysine acetylation. carboxyl terminus (cMED19a) harbors a mixed-charged intrinsically disordered region (MC-IDR) interaction liquid-liquid phase separation (LLPS). Plant human cMED19 are sufficient form heterotypic ORE1. Human MC-IDR, but not yeast IDR, partially complements med19a suggesting functional conservation evolutionarily distant eukaryotes. Phylogenetic analysis eukaryotic revealed MC-IDR could arise through convergent evolution. Our result MED19 suggests plant during stress