The eTM–miR858–MYB62‐like module regulates anthocyanin biosynthesis under low‐nitrogen conditions in Malus spectabilis

• DOI: 10.1111/nph.18894
• Publication Date: 2023-03-21T13:01:50Z
• Authors: Jiaxin Meng; Han Wang; Rufei Chi; Yuhang Qiao; Jun Wei; Yuqin Zhang; Meiling Han; Yu Wang; Houhua Li
• Keywords: Malus

Summary The anthocyanin content increases in Malus spectabilis leaves under low‐nitrogen conditions. Noncoding RNAs are indicated to play key regulatory roles biosynthesis. However, the functional of noncoding biosynthesis conditions remain elusive. In this study, miR858 was screened as a regulator through whole‐transcriptome sequencing. Then, we used an entry point explore network lncRNA–miRNA–mRNA by dual‐luciferase reporter assays and GUS histochemical staining assays, well identify mechanism both transient stable transformation experiments . MiR858 overexpression increased total content. acted negatively regulating its target gene, MsMYB62‐like , condition. inhibited expression MsF3′H thereby addition, eTM858‐1 eTM858‐2 were identified endogenous mimics that bind prevent cleavage regulate results clarify which eTM–miR858– MYB62‐like module regulates