SlERF.H6 mediates the orchestration of ethylene and gibberellin signaling that suppresses bitter‐SGA biosynthesis in tomato

• DOI: 10.1111/nph.19048
• Publication Date: 2023-06-08T06:15:56Z
• Authors: Yingchen Hao; Lijun Xiang; Jun Lai; Chun Li; Yue Zhong; Weizhen Ye; Jie Yang; Jun Yang; Shouchuang Wang
• Keywords: Glycoalkaloid; Crosstalk; Metabolic pathway

Summary Steroidal glycoalkaloids (SGAs) constitute a characteristic class of antinutritional metabolites that are found in certain Solanum species. Despite the considerable studies on SGA biosynthesis, mechanisms crosstalk between hormone signaling pathways regulate content still remain to be elucidated. Here, we performed metabolic genome‐wide association study (mGWAS) based levels and identified SlERF.H6 as negative regulator bitter‐SGA biosynthesis. repressed expression biosynthetic glycoalkaloid metabolism ( GAME ) genes caused subsequent decrease abundance bitter SGAs. Furthermore, were shown act downstream GAME9, biosynthesis tomato. We also uncovered interplay ethylene gibberellin (GA) regulating SlERF.H6, acting component signaling, modulated GA by inhibiting SlGA2ox12 expression. Increasing endogenous 12 53 SlERF.H6‐OE could inhibit Additionally, 1‐aminocyclopropane‐1‐carboxylic acid (ACC) treatment decreased stability weakening its inhibition , accumulation. Our findings reveal key role regulation through coordinated ethylene‐gibberellin signaling.