Alternative splicing of TaHSFA6e modulates heat shock protein–mediated translational regulation in response to heat stress in wheat

• DOI: 10.1111/nph.19100
• Publication Date: 2023-07-05T07:12:30Z
• Authors: Jingjing Wen; Zhen Qin; Lv Sun; Yumei Zhang; Dongli Wang; Huiru Peng; Yingyin Yao; Zhaorong Hu; Zhongfu Ni; Qixin Sun; Mingming Xin
• Keywords: Stress granule; Splicing factor; Polysome

Heat stress greatly threatens crop production. Plants have evolved multiple adaptive mechanisms, including alternative splicing, that allow them to withstand this stress. However, how splicing contributes heat responses in wheat (Triticum aestivum) is unclear. We reveal the shock transcription factor gene TaHSFA6e alternatively spliced response generates two major functional transcripts: TaHSFA6e-II and TaHSFA6e-III. TaHSFA6e-III enhances transcriptional activity of three downstream protein 70 (TaHSP70) genes a greater extent than does TaHSFA6e-II. Further investigation reveals enhanced due 14-amino acid peptide at its C-terminus, which arises from predicted form an amphipathic helix. Results show knockout or TaHSP70s increases sensitivity wheat. Moreover, are localized granule following exposure involved regulating disassembly translation re-initiation upon relief. Polysome profiling analysis confirms translational efficiency stored mRNAs lower recovery stage Tahsp70s mutants wild types. Our finding provides insight into molecular mechanisms by improves thermotolerance