ER body‐resident myrosinases and tryptophan specialized metabolism modulate root microbiota assembly

• DOI: 10.1111/nph.19289
• Publication Date: 2023-09-29T06:57:03Z
• Authors: Arpan Kumar Basak; Anna Piasecka; Jana Hucklenbroich; Gözde Merve Türksoy; Rui Guan; Pengfan Zhang; Felix Getzke; Ruben Garrido‐Oter; Stephane Hacquard; Kazimierz Strzałka; Paweł Bednarek; Kenji Yamada; Ryohei Thomas Nakano
• Keywords: 2. Zero hunger; Glycoside Hydrolases; Bacteria; Microbiota; Tryptophan; glucosinolate; root exudate; endoplasmic reticulum (ER) body; host–microbe interaction; Plant Roots; Soil; Rhizosphere; microbiota; specialized metabolism; Soil Microbiology

Endoplasmic reticulum (ER) bodies are ER-derived structures that contain a large amount of PYK10 myrosinase, which hydrolyzes tryptophan (Trp)-derived indole glucosinolates (IGs). Given the well-described role IGs in root-microbe interactions, we hypothesized ER roots important for interaction with soil-borne microbes at root-soil interface. We used mutants impaired (nai1), body-resident myrosinases (pyk10bglu21), IG biosynthesis (myb34/51/122), and Trp specialized metabolism (cyp79b2b3) to profile their root microbiota community natural soil, evaluate impact axenically collected exudates on soil or synthetic microbial communities, test response fungal endophytes mono-association setup. Tested exhibited altered bacterial communities rhizoplane endosphere, respectively. Natural soils treated mutant distinctive profiles from those wild-type (WT) exudates. Most tested severely restricted growth cyp79b2b3, part also pyk10bglu21. Our results suggest resident modulate root-secreted metabolites thereby influence root-microbiota interactions.