ER body‐resident myrosinases and tryptophan specialized metabolism modulate root microbiota assembly

2. Zero hunger Glycoside Hydrolases Bacteria Microbiota Tryptophan glucosinolate root exudate endoplasmic reticulum (ER) body host–microbe interaction Plant Roots Soil Rhizosphere microbiota specialized metabolism Soil Microbiology
DOI: 10.1111/nph.19289 Publication Date: 2023-09-29T06:57:03Z
ABSTRACT
Endoplasmic reticulum (ER) bodies are ER-derived structures that contain a large amount of PYK10 myrosinase, which hydrolyzes tryptophan (Trp)-derived indole glucosinolates (IGs). Given the well-described role IGs in root-microbe interactions, we hypothesized ER roots important for interaction with soil-borne microbes at root-soil interface. We used mutants impaired (nai1), body-resident myrosinases (pyk10bglu21), IG biosynthesis (myb34/51/122), and Trp specialized metabolism (cyp79b2b3) to profile their root microbiota community natural soil, evaluate impact axenically collected exudates on soil or synthetic microbial communities, test response fungal endophytes mono-association setup. Tested exhibited altered bacterial communities rhizoplane endosphere, respectively. Natural soils treated mutant distinctive profiles from those wild-type (WT) exudates. Most tested severely restricted growth cyp79b2b3, part also pyk10bglu21. Our results suggest resident modulate root-secreted metabolites thereby influence root-microbiota interactions.
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