FLOURY ENDOSPERM24, a heat shock protein 101 (HSP101), is required for starch biosynthesis and endosperm development in rice

• DOI: 10.1111/nph.19761
• Publication Date: 2024-04-18T06:50:05Z
• Authors: Hongming Wu; Yulong Ren; Hui Dong; Chen Xie; Lei Zhao; Xin Wang; Fulin Zhang; Binglei Zhang; Xiaokang Jiang; Yunshuai Huang; Ruonan Jing; Jian Wang; Rong Miao; Xiuhao Bao; Mingzhou Yu; Thanhliem Nguyen; Changling Mou; Yunlong Wang; Yihua Wang; Cailin Lei; Zhijun Cheng; Ling Jiang; Jianmin Wan
• Keywords: 0303 health sciences; 03 medical and health sciences

Summary Endosperm is the main storage organ in cereal grain and determines yield quality. The molecular mechanisms of heat shock proteins regulating starch biosynthesis endosperm development remain obscure. Here, we report a rice floury mutant flo24 that develops abnormal grains central starchy cells. Map‐based cloning complementation test showed FLO24 encodes protein HSP101, which localized plastids. mutated T296I dramatically lost its ability to hydrolyze ATP rescue thermotolerance defects yeast hsp104 mutant. more severe when grown under high‐temperature conditions than normal conditions. And was induced at high temperature. physically interacts with several key enzymes required for biosynthesis, including AGPL1, AGPL3 PHO1. Combined biochemical genetic evidence suggests acts cooperatively HSP70cp‐2 regulate rice. Our results reveal as an important regulator development, might function maintaining activities involved