ZmSPL10, ZmSPL14 and ZmSPL26 act together to promote stigmatic papilla formation in maize through regulating auxin signaling and ZmWOX3A expression

• DOI: 10.1111/nph.19961
• Publication Date: 2024-07-16T06:06:22Z
• Authors: Dexin Kong; Yifeng Jing; Yaping Duan; Mengqi He; Hui Ding; Heying Li; Zhuojun Zhong; Zhigang Zheng; Xiuying Fan; Xuan Pan; Yanqun Li; Mei Bai; Xinjian Li; Minhua Luo; Weicong Xue; Xiaoming Zhang; Xin Xu; Yateng Yuan; Ting Zou; Lihong Chen; Wenyan Ding; Yongping Zhao; Baobao Wang; Hong Wu; Qing Liu; Haiyang Wang
• Keywords: Major duodenal papilla; Dermal papillae

Summary Maize silk is a specialized type of stigma, covered with numerous papillae for pollen grain capture. However, the developmental process stigmatic and underlying regulatory mechanisms have remained largely unknown. Here, we combined cytological, genetic molecular studies to demonstrate that three homologous genes ZmSPL10 , ZmSPL14 ZmSPL26 play central role in promoting papilla formation maize. We show their triple knockout mutants are nearly complete lack papilla, resulting severe reduction kernel setting. Cellular examination reveals developed from precursor cell, which smaller daughter cell asymmetric division epidermal cell. In situ hybridization shows target SPI1 ZmPIN1b ZmARF28 ZmWOX3A preferentially expressed cells papillae. Moreover, ZmSPL10, directly bind promoters promote expression. Further, Zmwox3a display defects reduced seed Collectively, our results act together development through regulating auxin signaling