Plant‐to‐plant defence induction in cotton is mediated by delayed release of volatiles upon herbivory

• DOI: 10.1111/nph.20202
• Publication Date: 2024-10-17T11:26:33Z
• Authors: Luca Grandi; Wenfeng Ye; Mary V. Clancy; Armelle Vallat; Gaétan Glauser; Luis Abdala‐Roberts; Thierry Brevault; Betty Benrey; Ted C. J. Turlings; Carlos Bustos‐Segura
• Keywords: H01 - Protection des végétaux - Considérations générales; 570; composé volatil; plant defence; F60 - Physiologie et biochimie végétale; défense chimique; herbivore; Gossypium hirsutum; http://aims.fao.org/aos/agrovoc/c_29779; [SDV.EE]Life Sciences [q-bio]/Ecology; http://aims.fao.org/aos/agrovoc/c_e2805193; plant signalling; http://aims.fao.org/aos/agrovoc/c_3567; herbivory-induced plant volatiles (HIPVs); mécanisme de défense; 580; [SDV.EE]Life Sciences [q-bio]/Ecology, environment; Gossypium; volatile organic compounds (VOCs); Research; http://aims.fao.org/aos/agrovoc/c_3339; http://aims.fao.org/aos/agrovoc/c_24933; http://aims.fao.org/aos/agrovoc/c_16371; préférence alimentaire; gossypol; [SDV.EE] Life Sciences [q-bio]/Ecology, environment; defence induction; plant-plant communication; http://aims.fao.org/aos/agrovoc/c_35269; http://aims.fao.org/aos/agrovoc/c_3335; environment

Summary Caterpillar feeding immediately triggers the release of volatile compounds stored in the leaves of cotton plants. Additionally, after 1 d of herbivory, the leaves release other newly synthesised volatiles. We investigated whether these volatiles affect chemical defences in neighbouring plants and whether such temporal shifts in emissions matter for signalling between plants. Undamaged receiver plants were exposed to volatiles from plants infested with Spodoptera caterpillars. For receiver plants, we measured changes in defence‐related traits such as volatile emissions, secondary metabolites, phytohormones, gene expression, and caterpillar feeding preference. Then, we compared the effects of volatiles emitted before and after 24 h of damage on neighbouring plant defences. Genes that were upregulated in receiver plants following exposure to volatiles from damaged plants were the same as those activated directly by herbivory on a plant. Only volatiles emitted after 24 h of damage, including newly produced volatiles, were found to increase phytohormone levels, upregulate defence genes, and enhance resistance to caterpillars. These results indicate that the defence induction by volatiles is a specific response to de novo synthesised volatiles, suggesting that these compounds are honest signals of herbivore attack. These findings point to an adaptive origin of airborne signalling between plants.