Arabidopsis perceives caterpillar oral secretion to increase resistance by reactive oxygen species‐enhanced glucosinolate hydrolysis

• DOI: 10.1111/nph.70014
• Publication Date: 2025-03-11T15:21:36Z
• Authors: Xueying Chen; Man‐Ni Wu; Qiu‐Yi Chen; Pai Li; Mu‐Yang Wang; Jiancai Li; Xiu‐Fang Xin; Ying‐Bo Mao
• Keywords: Respiratory burst; Brassicaceae; Pseudomonas syringae

In Arabidopsis, the outbreaks of reactive oxygen species (ROS) occur upon pathogen recognition by pattern- and effector-triggered immunity (PTI ETI, respectively), which plays a significant role in disease resistance. Here, we found that Arabidopsis also experiences two ROS (oral secretion (OS)-induced (ROSOS)) perception OS from cotton bollworm (Helicoverpa armigera) other lepidopterans. Oral secretion-induced burst requires PTI machinery, including BRI1-ASSOCIATED RECEPTOR KINASE1 (BAK1) BOTRYTIS-INDUCED (BIK1). are primarily produced respiratory oxidase homologue D (RBOHD) apoplast, double mutant, rbohdf, exhibits reduced resistance to Insect biting rather than wounding induces gene expressions plant defense-associated toxin catabolic processes, facilitating breakdown leaf glucosinolates into bioactive intermediates, like sulforaphane, thereby impeding insect herbivory. Our investigation demonstrates perceives BAK1-BIK1-dependent manner employs RBOHD produce enhancing its accelerating glucosinolate hydrolysis.