Characterization of mesenchymal progenitor cells in crown and root pulp from human mesiodentes

• DOI: 10.1111/odi.12234
• Publication Date: 2014-03-08T13:22:05Z
• Authors: M Sato; T Toriumi; N Watanabe; E Watanabe; D Akita; T Mashimo; Y Akiyama; K Isokawa; T Shirakawa; MJ Honda
• Keywords: Male; Tooth Crown; Adolescent; Reverse Transcriptase Polymerase Chain Reaction; Mesenchymal Stem Cells; Flow Cytometry; Colony-Forming Units Assay; Young Adult; 03 medical and health sciences; 0302 clinical medicine; Tooth, Supernumerary; Child, Preschool; Antigens, Surface; Humans; Female; Tooth Root; Child; Dental Pulp

Objective Mesiodentes are usually found in the central position of upper or lower jaw as supernumerary teeth. Here, we obtained 10 mesiodentes and three permanent teeth ( PT ) separated dental pulp DP from these into crown root portions. We then characterized compared isolated portion‐derived cells (crown cells) with (root using a range vitro assays. Materials methods Crown were examined for cell surface marker expression, colony‐forming unit‐fibroblast CFU ‐F), proliferation, cycle characteristics markers, osteogenic adipogenic differentiation. Results The proportion CD 105‐positive 105 + vs varied among mesiodentes, but not . When there more than cells, showed higher ‐F, proliferation capacity, differentiation capacity. In contrast, when contained addition, sorted ‐F capacity − cells. Conclusion These results indicated that is an effective means characterizing ‐derived mesiodentes.