A three‐dimensional analysis of primary failure of eruption in humans and mice

• DOI: 10.1111/odi.13249
• Publication Date: 2019-12-05T06:20:35Z
• Authors: Nicha Tokavanich; Aditi Gupta; Mizuki Nagata; Akira Takahashi; Yuki Matsushita; Marilia Yatabe; Antonio Ruellas; Lucia Cevidanes; Koutaro Maki; Tetsutaro Yamaguchi; Noriaki Ono; Wanida Ono
• Keywords: Primary (astronomy); Tooth eruption

Primary failure of eruption (PFE) is a genetic disorder exhibiting the cessation tooth eruption. Loss-of-function mutations in parathyroid hormone (PTH)/parathyroid hormone-related peptide (PTHrP) receptor (PTH/PTHrP receptor, PPR) were reported as underlying cause this humans. We showed PFE mouse model that PTHrP-PPR signaling responsible for normal dental follicle cell differentiation and However, mechanism defect remains undefined. In descriptive study, we aim to chronologically observe root formation molars through 3D microCT analyses.Two individuals with recruited at Showa University. A was generated by deleting PPR specifically PTHrP-expressing divided into three groups, PPRfl/fl ;R26RtdTomato/+ (Control), PTHrP-creER;PPRfl/+ (cHet), PTHrP-creER;PRRfl/fl (cKO).Images from human subjects acquired CBCT. All groups samples studied postnatal days 14, 25, 91, 182 after tamoxifen pulse P3, superimposition images among rendered.Mouse exhibited similar presentation CT analyses. The quantitative analysis mice demonstrated statistically significant decrease height cKO first second compared other day 25. Additionally, significantly shortened roots dilacerations associated reduced interradicular bone height.Mouse erupt much slower rate molars, dilacerated defective bones.