Benzo[a]pyrene promotes progression in tongue squamous cell carcinoma

• DOI: 10.1111/odi.13489
• Publication Date: 2020-06-19T02:30:25Z
• Authors: Linxuan Huang; Xiaofen Xiao; Yupeng Yao; Jianping Yu; Qingjian Chen; Puping Liang; Yan Zhang
• Keywords: 0301 basic medicine; Mice; 03 medical and health sciences; Tongue; Cell Movement; Cell Line, Tumor; Benzo(a)pyrene; Carcinoma, Squamous Cell; Animals; Tongue Neoplasms

Abstract Objectives Benzo[a]pyrene (B[ a ]P) is member of the polycyclic aromatic hydrocarbon (PAH) family. Although potent carcinogenicity high‐dose B[ ]P has been extensively reported, effects long‐term exposure to on progression tongue squamous cell carcinoma (TSCC) are poorly understood. Methods In present study, TSCC cells were treated with 5 or 50 nM for three months. The proliferation and chemoresistance ]P‐treated 5‐fluorouracil cisplatin detected by CCK8. motility was evaluated wound healing analysis, invasion assay, three‐dimensional culture in decellularized mouse matrix. Xenograft assay used investigate aggressiveness cells. Immunofluorescence staining, terminal restriction fragment whole‐genome sequence determine mutation spectrums. Results Long‐term exhibited increased cisplatin. addition, data from sequencing demonstrated that C:T A:T transitions predominant nucleotide substitutions occurred CAL27 Furthermore, 102 non‐synonymous stop‐gain mutations enriched extracellular‐matrix‐receptor interactive pathway. Conclusions may contribute genomic instability, therefore, promote TSCC.