miR‐450b promotes cell migration and invasion by inhibiting SERPINB2 in oral squamous cell carcinoma

• DOI: 10.1111/odi.14407
• Publication Date: 2022-10-17T16:25:50Z
• Authors: Xiaotang Wang; Xiaocui Nie; Guoqiang Xu; Jiping Gao; Binhong Wang; Junting Yang; Guohua Song
• Keywords: Hyperplasia; Squamous Cell Carcinoma of Head and Neck; Carcinogenesis; 3. Good health; Gene Expression Regulation, Neoplastic; MicroRNAs; 03 medical and health sciences; Cell Transformation, Neoplastic; 0302 clinical medicine; Cell Movement; Head and Neck Neoplasms; Cell Line, Tumor; Carcinoma, Squamous Cell; Animals; Humans; Mouth Neoplasms; RNA, Small Interfering; Cell Proliferation

Abstract Objective microRNA‐450b ( miR‐450b ) plays an important role in cancer progression; however, its function oral squamous cell carcinoma (OSCC) remains largely unknown. This study aimed to investigate the action mechanisms of OSCC. Materials and Methods OSCC animal model was established via continuous induction with single‐drug 7, 12‐dimethylbenzo[a]anthracene (DMBA). Animal tissue samples were pathologically typed using haematoxylin–eosin (HE) staining. The Cancer Genome Atlas (TCGA) database used predict SERPINB2 expression head neck (HNSCC). qRT‐PCR Western blotting detect gene protein cells, respectively. proliferation, growth, migration invasion detected CCK‐8, colony formation, transwell matrigel assays, Bioinformatic tools target genes. Dual‐luciferase reporter assay verify targeting between . Finally, small interfering RNA (siRNA) reduce SERPINB 2 effect on tumourigenesis. Results Four stages carcinogenesis (normal epithelium, simple epithelial hyperplasia, dysplasia OSCC) identified. found be overexpressed samples, HNSCC human cells. Upregulation significantly promoted invasion, while downregulation had opposite effect. a gene, negatively correlated expression. Altering effectively inhibited metastasis epithelial‐mesenchymal transition (EMT). Conclusions key tumourigenesis by regulating migration, EMT