Effects of Integrin‐Linked Kinase Silencing Combined With Trichostatin A on Cancer Stem Cells

• DOI: 10.1111/odi.15377
• Publication Date: 2025-05-14T05:14:36Z
• Authors: Yulu Wu; Zhiyong Guo; Zhangao Li; Chenchen Cai; Jiyuan Liu; Xiufa Tang; Lin Que
• Keywords: Trichostatin A; Integrin-linked kinase

ABSTRACT Objectives Oral squamous cell carcinoma (OSCC) is characterized by high invasiveness and metastasis, with cancer stem cells (CSCs) playing a central role in tumor progression. This study investigates the effects of integrin‐linked kinase (ILK) silencing trichostatin A (TSA) treatment on CSCs, assessing their potential to diminish CSC properties inhibit OSCC Methods Materials CSCs were enriched isolated from primary samples Tca8113 line MOC1 using side population (SP) analysis, characteristics therapeutic impact treatments assessed through assays such as MTT, wound healing, invasion, cycle, apoptosis. Results Higher SP content correlated significantly poor pathological classification, recurrence. Treated showed reduced proliferation, migration, along increased In vivo experiments demonstrated that combined substantially growth. Conclusion The confirms efficacy targeting ILK TSA OSCC, suggesting promising strategy for CSC‐directed therapies merit further investigation.