Co‐expression of Arabidopsis transcription factor, AtMYB12, and soybean isoflavone synthase, GmIFS1, genes in tobacco leads to enhanced biosynthesis of isoflavones and flavonols resulting in osteoprotective activity

• DOI: 10.1111/pbi.12118
• Publication Date: 2013-10-08T14:01:05Z
• Authors: Ashutosh Pandey; Prashant Misra; Mohd P. Khan; Gaurav Swarnkar; Mahesh C. Tewari; Sweta Bhambhani; Ritu Trivedi; Naibedya Chattopa...; Prabodh K. Trivedi
• Keywords: MYB

Summary Isoflavones, a group of flavonoids, restricted almost exclusively to family Leguminosae are known exhibit anticancerous and anti‐osteoporotic activities in animal systems have been target for metabolic engineering commonly consumed food crops. Earlier efforts based on the expression legume isoflavone synthase ( IFS ) genes nonlegume plant species led limited success terms content transgenic tissue due limitation substrate enzyme. In this work overcome limitation, activation multiple flavonoid pathway using Arabidopsis transcription factor At MYB 12 has carried out. We developed tobacco lines constitutively co‐expressing Gm 1 (soybean or independently out their phytochemical molecular analyses. The leaves were found elevated flavonol along with accumulation substantial amount genistein glycoconjugates being at highest levels that could be engineered till date. Oestrogen‐deficient (ovariectomized, Ovx) mice fed leaf extract from but not wild‐type exhibited significant conservation trabecular microarchitecture, reduced osteoclast number osteoclastogenic genes, higher total serum antioxidant increased uterine oestrogenicity compared Ovx treated vehicle (control). skeletal effect was comparable oestrogen‐treated mice. Together, our results establish an efficient strategy successful isoflavones other flavonoids crop plants provide direct evidence improved osteoprotective extract.